One-step purification of nisin A by immunoaffinity chromatography.
نویسندگان
چکیده
The lantibiotic nisin A was purified to homogeneity by a single-step immunoaffinity chromatography method. An immunoadsorption matrix was developed by direct binding of anti-nisin A monoclonal antibodies to N-hydroxysuccinimide-activated Sepharose. The purification procedure was rapid and reproducible and rendered much higher final yields of nisin than any other described method.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 63 12 شماره
صفحات -
تاریخ انتشار 1997